General Cell Lines and Cell Cultures
ATCC Streptococcus mitis; NS 51; genomic DNA
Genomic DNA from Streptococcus mitis strain NCTC 12261 (ATCC 49456). This product can be used in PCR and other molecular biology applications.
Biochain Institute Inc Matched Pair - Total Protein - Human Primary Tumor and Metastatic Tumor Tissue (PM): Stomach, 0.2 x2 mg /PK
BioChain's human total protein matched pair products include: Primary Pair (PP), Primary and Metastatic Pair (PM). PP consists of total protein isolated from primary tumor and its adjacent normal tissue; PM consists of total protein from primary tumor andcorresponding metastastic tumor. Total proteins in each pair are prepared from the same donor. This product line is designedfor identifying tumor-specific genes and tumor metastasis genes.
Eurofins Discoverx PathHunter CHO-K1 mOPRD1 β-Arrestin Cell Line, 2 vials. (93-0611C2)
The PathHunter mOPRD1 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. DADLE) based activation of mOPRD1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mOPRD1 and EA-tagged β-Arrestin-2. Activation of mOPRD1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx ChemiScreen α1B Adrenergic Receptor Membrane Preparation, 1 vial. (HTS158M)
The ChemiScreen α1B Adrenergic Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of ADRA1B surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
ATCC Kasumi-1; Leukemia (AML); Human (Homo sapiens)
Kasumi-1 is a myeloblast cell that was isolated from the peripheral blood of an acute myeloblastic leukemia Asian male patient. This cell line was deposited by H Asou in 1989.
ATCC 4T1; Mammary Tumor; Mouse (Mus musculus)
The tumor growth and metastatic spread of 4T1 cells in BALB/c mice very closely mimic human breast cancer. This tumor-derived cell line is an animal model for stage IV human breast cancer. 4T1-induced tumors can be used as a post-operative model as well as a nonsurgical model because the 4T1-induced tumor metastasizes spontaneously in both models with similar kinetics.
ATCC Primary Umbilical Vein Endothelial Cells; Normal, Human
Normal Primary Human Umbilical Vein Endothelial Cells (HUVEC), when grown in Vascular Cell Basal Media supplemented with Endothelial Cell Growth Kit components, provide an ideal cell system to propagate HUVEC in low serum conditions with or without the addition of human recombinant VEGF.
Eurofins Discoverx PathHunter CHO-K1 GPR109B β-Arrestin Cell Line, 2 vials. (93-0791C2)
The PathHunter GPR109B cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. 3-hydroxyoctanoic acid) based activation of GPR109B GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR109B and EA-tagged β-Arrestin-2. Activation of GPR109B stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CRHR2 Activated GPCR Internalization U2OS Cell Line, 2 vials. (93-0752C3)
The PathHunter CRHR2 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of CRHR2 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CRHR2. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CRHR2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter EDG5 Activated GPCR Internalization U2OS Cell Line, 2 vials. (93-0735C3)
The PathHunter EDG5 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of EDG5 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged EDG5. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting EDG5-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 ADRB2 β-Arrestin Cell Line, 2 vials. (93-0182C2)
The PathHunter ADRB2 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. isoproterenol) based activation of ADRB2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged ADRB2 and EA-tagged β-Arrestin-2. Activation of ADRB2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CRHR1 Activated GPCR Internalization U2OS Cell Line, 2 vials. (93-0726C3)
The PathHunter CRHR1 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of CRHR1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CRHR1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CRHR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC Adipose-Derived Mesenchymal Stem Cells; Normal, Human
Normal Human Adipose-Derived Mesenchymal Stem Cells are fibroblast-like cells that, when grown in Mesenchymal Stem Cell Basal Media supplemented with Mesenchymal Stem Cell Growth Kit Low serum components, provide an ideal cell system to propagate mesenchymal stem cells in low serum (2% FBS) conditions. When maintained under optimal growth conditions, ATCC Normal Human Adipose-Derived Mesenchymal Stem Cells have been shown to be multipotent, capable of differentiating down the adipogenic, osteogenic, and chondrogenic lineages. The cells are cryopreserved at the second passage to ensure the highest viability and plating efficiency. ATCC Primary Cell Solutions cells, media, supplements, and reagents are quality tested together to guarantee optimum performance and reliability.
ATCC MC3T3-E1 Subclone 4; Preosteoblast; Mouse (Mus musculus)
MC3T3-E1 Subclone 4 preosteoblasts were isolated from the cloned but phenotypically heterogeneous MC3T3-E1 cell line. This subclone was selected for high osteoblast differentiation and mineralization after growth in medium containing ascorbic acid. This cell line is a good model for studying in vitro osteoblast differentiation, particularly ECM signaling. It's behavior is similar to primary calvarial osteoblasts.